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Updated 05 October 2026 · Source: CBSE board papers and marking schemes
Free · Class 12 Biology · 11 questions

Biotechnology & its Applications: Class 12 Previous Year Questions

Every CBSE board exam question on Biotechnology & its Applications (Class 12 Biology) from 2024 to 2026, with the years it was asked and the official marking-scheme answer in the free app.

Key facts

Biotechnology & its Applications questions asked in CBSE board exams

Repeated questions first. Open the free app to see each question as it appeared in the paper, with its marking scheme.

  1. _________ Page 15 of 24 { } 27. A farmer grew two varieties of corn crop in field A and B. He grew normal corn crops in field A and GM corn crops in field B. He observed that corn borers attacked only in field A. To control it, spores of Bt were sprayed on fie…
    Board 20263 marksshort answer
  2. Assertion (A) : The milk produced by transgenic cow ‘Rosie’ was nutritionally more balanced product for human babies than natural cow milk. Reason (R) : It was human protein enriched milk containing human alpha lactaglobulin.
    Board 20261 markAssertion-Reason
  3. (a) The insulin synthesised in our body is different from that synthesised by Eli Lilly company using recombinant DNA technology. Differentiate between them. (b) Why the insulin extracted from an animal source is not in use these days ?
    Board 20253 marksshort answer
  4. (a) (i) Why should a cell be made competent to take up an alien DNA ? How can a bacterial cell be made competent using calcium ions ? Explain. 2 (ii) (1) State the importance of gel electrophoresis in biotechnology. (2) Explain the principle on which this tech…
    Board 20245 markslong answer
  5. (a) (i) What are transgenic animals ? 1 (ii) Name the first transgenic cow and state its importance. 2 OR (b) (i) Explain the convention for naming EcoRI. 2 (ii) With the help of an illustration only, show the action of EcoRI on a DNA Polynucleotide.
    Board 20243 marksshort answer
  6. The picture given below shows : 3 (a) Roots of a typical control tobacco crop plant (infected). (b) Transgenic tobacco plant showing healthy roots even after deliberate infection by nematode. Explain how this transformation was achieved in the tobacco plant. (…
    Board 20243 marksshort answer
  7. Which one of the following transgenic animals is being used to test the safety of the polio vaccine ? (A) Sheep (B) Goat (C) Pig (D) Mice
    Board 20241 markMCQ
  8. The improved trait that is found in the genetically modified transgenic crop Golden rice is : (A) High lysine content (B) Insect resistant (C) High protein content (D) High vitamin-A content
    Board 20241 markMCQ
  9. Bt cotton is known to be a pest resistant GM crop. How does Bt cotton get its insect resistance? [2 marks]
    CBSE practice 2021-225 markslong answer
  10. In certain diseased conditions such as pneumonia, the fingernails of an individual turn blue. What could be the reason for this? A. blood does not reach fingernails due to an increase in fat content around the fingernails B. nails become cold due to a decrease…
    CBSE practice 2022-231 markMCQ
  11. Assertion (A): Gene therapy is a method of treating a disorder but cannot cure it. Reason (R): Cells are drawn from a patient and the functional gene is introduced into these cells and transferred back to the patient.
    CBSE practice 2022-231 markAssertion-Reason

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Frequently asked questions

How many marks does Biotechnology & its Applications carry in the CBSE Class 12 Biology board exam?

Across recent CBSE board papers, Biotechnology & its Applications carried about 2.5 marks per paper on average, making it number 11 of 13 chapters by weightage.

Which years were Biotechnology & its Applications questions asked in CBSE boards?

Questions from Biotechnology & its Applications appeared in the 2024, 2025, 2026 CBSE Class 12 board exams.

Where can I practise Biotechnology & its Applications previous year questions for free?

On BoardPrep: all 11 Biotechnology & its Applications questions are free to practise online, with official CBSE marking-scheme answers where published.